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KMID : 0357319950300040425
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Journal of the Korean Society for Microbiology
1995 Volume.30 No. 4 p.425 ~ p.430
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Quantitation of Human Cytomegalovirus(HCMV) by Enzyme Immunoassay
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황응수
이미우/박재원/임동균/서주영/박정규/국윤호/최성배/차창룡
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Abstract
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The rapid quantitative method for the detection of HCMV is required to evaluate the clinical state for HCMV infections. We therefore attempted to develop enzyme-linked immunosorbent assay for the detection of HCMV in culture supernatant and urine
by
using monoclonal antibody(MCMVA 135) specific to HCMV glycoprotein B(gB). MCMVA 135 was purified by salting-out, and was attached to the plate. The culture supernatant of HCMV infected cells or urine from HCMV infected patient were applied as
antigens,
and then the peroxidase-conjugated MCMVA 135 was added. After the substrate was added the absorbance was measured at 405nm. The standard curve showed that HCMV in culture supernatant could be detected from 3×10E3 PFU/ml to 5×10E4 PFU/ml
linearly.
HCMV was not detected in all but one urine sample from eight healthy control persons whereas detected from at the concentration of 3×10E3 PFU/ml to 5×10E4 PFU/ml in all but one urine sample from eight HCMV-infected patients. These results
revealed
that the specificity and the sensitivity were 87%, respectively.
This detection method of HCMV antigen from the culture supernatant and urine would offer advantages in terms of quantitative sensitivity.
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KEYWORD
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